Development and Validation of Simple Uv-spectrophotometric Method for Quantitation of Bromazepam in Api and Solid Dosage Formulation

Bromazepam are a well-known class of psychoactive drugs; they are known primarily for their hypnotic and sedative effects. In this recent research a very simple, precise, least time consuming, versatile, inexpensive and efficient UV Spectrophotometric method developed for the quantitation of Bromazepam in Active Pharmaceutical Ingredient and its pharmaceutical solid dosage formulation. The assay is based on the Ultraviolet Spectroscopy (UV), measuring max at 285 nm for bromazepam. A sample of bulk was dissolved in 0.1N methanolic sulphuric acid to produce a desired concentration of solution containing bromazepam. Similarly, a sample of pharmaceutical formulation was grind and dissolved in 0.1N methanolic sulphuric acid and various dilutions were made. The absorbance of bulk, dosage formulation preparation was measured at 285nm against 0.1 N methanolic sulphuric acid (blank). Calibration curves were linear over the range of 5-40 μgmL -1 with a correlation coefficient is ≥ 0.9996 at max 285 nm. The analysis shows a linear relationship between the absorbance and concentration. The proposed method was statistically validated and successfully applied for routine analysis of bromazepam in bulk and tablet dosage forms.